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《中国美容医学》[ISSN:1008-6445/CN:61-1347/R]

卷:

期数:

2023年6期

页码:

94-98

栏目:

出版日期:

2023-06-10

文章信息/Info

Title:

Effi cacy of PD-1 Antibody Regulating the Balance of CD8+ T Cells and Treg Cells in Diabetic Rats and Promoting the Healing of Diabetic Foot Ulcers

文章编号:

1008-6455（2023）06-0094-04

作者:

黄亚莲¹ ; 刘 莹² ; 赖舒畅¹ ; 符茂雄¹

(1.海南医学院第二附属医院内分泌科 海南 海口 570311 ;2.海南医学院药物安评中心 海南 海口 570216 )

Author(s):

HUANG Yalian¹ ; LIU Ying² ; LAI Shuchang¹ ; FU Maoxiong¹

( 1.Department of Endocrinology,the Second Affi liated Hospital of Hainan Medical College,Haikou 570311,Hainan ,China; 2.Drug Safety Assessment Center of Hainan Medical College,Haikou 570216,Hainan,China)

关键词:

糖尿病足溃疡; PD-1抗体; CD8+ T细胞; 调节性T细胞; 肿瘤坏死因子-α; 白细胞介素-1β

Keywords:

diabetic foot ulcer;  PD-1 antibody;  CD8+ T cells;  Treg cells;  tumor necrosis factor-;  interleukin-1

分类号:

R587.2

文献标志码:

A

摘要:

目的：探讨PD-1抗体治疗糖尿病大鼠足溃疡的疗效及其作用机制。方法：将30只6～8周龄健康Sprague-Dawley大鼠随 机分为对照组、模型组和治疗组，每组10只，三组大鼠足部人为造成溃疡创口。采用流式细胞术计数三组大鼠脾脏中CD8+ T 细胞和调节性T细胞；Image J对创口照片进行分析计算伤口愈合率；HE染色和组织病理学分析溃疡创口处皮肤愈合情况； ELISA 测定大鼠足部溃疡创口组织中TNF-α和IL-1β的水平。结果：与对照组相比，模型组大鼠CD8+ T细胞计数明显下调， Treg 细胞计数明显升高（P＜0.05）；模型组大鼠足部溃疡创口愈合不佳，没有形成完整的新生表皮组织和肉芽组织，未见 胶原沉积；模型组大鼠创口处组织中TNF-α和IL-1β的水平明显升高（P＜0.05）。与模型组相比，治疗组大鼠CD8+ T细胞计 数明显回升，Treg细胞计数明显回落（P＜0.05）；治疗组大鼠足部溃疡创口愈合良好，有较为完整的新生表皮组织、肉芽 组织和一定的胶原沉积；治疗组大鼠创口处组织中TNF-α和IL-1β的水平明显降低（P＜0.05）。结论：应用人源化PD-1抗 体治疗糖尿病大鼠足溃疡具有明显的疗效。

Abstract:

Objective To investigate the effi cacy and mechanism of PD-1 antibody in the treatment of diabetic foot ulceration in rat model. Methods 30 Rats were randomly divided into control group, model group and treatment group, each group has 10 rats, artifi cial ulcer wounds on the feet of three groups of rats. Flow cytometry was used to count CD8+ T cells and regulatory T cells (Treg) cells in the spleens of the three groups of rats. Image J was used to analyze wound photos and calculate wound healing rates. HE staining and histopathological analysis was used to analyze ulcer wounds healing. ELISA was used to determine the levels of TNF-α and IL-1β in rats foot ulcer wound tissues. Results Compared with the control group, the CD8+ T cell count in the model group was signifi cantly down-regulated, and the Treg cell count was signifi cantly increased (P ＜0.05). The wounds of the foot ulcers of the rats in the model group did not heal well with no complete structure of new epidermal tissue and granulation tissue were formed, and no collagen deposition was found. The levels of TNF-α and IL-1β in the wound tissue of rats in the model group were signifi cantly increased (P ＜0.05). Compared with the model group, the CD8+ T cell count in the treatment group increased signifi cantly, and the Treg cell count decreased signifi cantly (P ＜0.05). The wounds of the foot ulcers of the rats in the treatment group healed well, with relatively complete structure of new epidermal tissue and granulation tissue were formed, and certain collagen deposition was found. The levels of TNF-α and IL-1β in the wound tissues of the rats in the treatment group were signifi cantly decreased (P ＜0.05). Conclusion The application of humanized PD-1 antibody in the treatment of diabetic rat foot ulcer has good curative effi cacy.

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更新日期/Last Update: 2023-07-10