[1]尹小芳,秦 岭,杨 超,等.ADSCs、VEGF和GM-CSF修复成纤维细胞放射性损伤的机制研究[J].中国美容医学,2023,(8):30-34.[doi:10.15909/j.cnki.cn61-1347/r.005846]
　YIN Xiaofang,QIN Ling,YANG Chao,et al.Study on the Mechanism of ADSCs, VEGF and GM-CSF in Repairing Radiation Damage of Fibroblasts?/html>[J].Medical Aesthetics and Beauty,2023,(8):30-34.[doi:10.15909/j.cnki.cn61-1347/r.005846]
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ADSCs、VEGF和GM-CSF修复成纤维细胞放射性损伤的机制研究()
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《中国美容医学》[ISSN:1008-6445/CN:61-1347/R]

卷:
期数:
2023年8期
页码:
30-34
栏目:
出版日期:
2023-08-10

文章信息/Info

Title:
Study on the Mechanism of ADSCs, VEGF and GM-CSF in Repairing Radiation Damage of Fibroblasts?/html>
文章编号:
1008-6455（2023）08-0029-05
作者:
尹小芳1 ; 秦 岭1 ; 杨 超3 ; 阎海萍4 ; 栗颖利2 ; 李 敏1
(1.解放军第960医院核医学科 山东 济南 250031 ;2.解放军第960医院烧伤整形科 山东 济南 250031 ;3.中国人民解放军海 军特色医学中心整形外科 上海 200000 ;4.济南军区第十一离职干部休养所 山东 济南 250000 )
Author(s):
YIN Xiaofang1 ; QIN Ling1 ; YANG Chao3 ; YAN Haiping4 ; LI Yingli2 ; LI Min1
( 1.Department of Nuclear Medicine of the 960th Hospital of the PLA,Jinan 250031,Shandong,China; 2.Department of Burn and Plastic Surgery, 960th Hospital of the People’s Liberation Army,Jinan 250031,Shandong,China; 3.People’s Liberation Army Navy Special Medical Center Plastic Surgery,Shanghai 200000,China; 4.Jinan Military Region 11th Retired Cadre Rest Center,Jinan 250000,Shandong,China)
关键词:
脂肪干细胞; 成纤维细胞; 辐射; 粒细胞-巨噬细胞集落刺激因子; 血管内皮生长因子
Keywords:
adipose-derived stem cells;  fibroblasts;  radiation;  Granulocyte-macrophage colony-stimulating factor;  Vascular endothelial growth factor
分类号:
R622
DOI:
10.15909/j.cnki.cn61-1347/r.005846
文献标志码:
A
摘要:
目的：研究对比脂肪干细胞（Adipose-derived stem cells，ADSCs）、血管内皮生长因子（Vascular endothelial growth factor，VEGF）和粒细胞-巨噬细胞集落刺激因子（Granulocyte-macrophage colony stimulating factor，GMCSF）对放射后成纤维细胞（Fibroblasts，Fb）修复的影响机制。方法：使用剂量为8 Gy的X线放射源，对大鼠的成纤维细 胞进行单次X线照射，建立ADSCs与放射后成纤维细胞的共培养模型。分别设置单纯放射后成纤维细胞为Fb2组，ADSCs与放 射后成纤维细胞共培养为Fb3组，VEGF+放射后成纤维细胞为VEGF组，GM-CSF+放射后成纤维细胞为GM-CSF组。通过VEGF、 GM-CSF 、ADSCs分别干预放射损伤的成纤维细胞，观察细胞凋亡、增殖、细胞周期及Western-Blot检测信号通路激活情况验 证结果。结果：VEGF、GM-CSF、ADSCs分别作用于放射损伤的成纤维细胞，可以促进细胞增殖、抑制细胞凋亡，加快细胞周 期由G2/M向G1/S进展，与照射组相比差异有统计学意义（P＜0.05），其程度强弱依次为ADSCs、GM-CSF、VEGF。VEGF组和 GM-CSF组p-Akt蛋白和p-Erk蛋白表达较照射组上调，差异有显著统计学意义（P＜0.01）；相同浓度ADSCs、GM-CSF和VEGF 促进p-Akt蛋白和p-Erk蛋白表达，作用程度强弱依次为ADSCs、GM-CSF、VEGF，差异有显著统计学意义（P＜0.01）。磷脂 酰肌醇-3-激酶/蛋白激酶B（Phosphatidylinositol-3-kinase/protein kinase B，PI3K-Akt）通路和丝裂原激活蛋白激酶 （Mitogen activated protein kinase，MAPK）通路可能发挥着重要作用，比如在VEGF和GM-CSF作用于ADSCs治疗放射损伤 后成纤维细胞过程中。ADSCs修复放射损伤后成纤维细胞的效果明显好于VEGF和GM-CSF单独修复放射损伤后成纤维细胞，表 明可能还有其他的潜在作用因子。结论：ADSCs、VEGF和GM-CSF干预的放射损伤后成纤维细胞主要通过增强细胞增殖、抑制 细胞凋亡及加快细胞周期由G2/M向G1/S发展。
Abstract:
Objective To discuss the eff ect mechanism of adipose-derived stem cells(ADSCs) on irradiation fi broblasts (Fb) through vascular endothelial growth factor (VEGF) and granulocyte-macrophage colony stimulating factor (GM-CSF). Methods Rats Fb were irradiated with a dose of 8 Gy X-ray source, and the co-culture of ADSCs and post-irradiated Fb was established. ADSCs were co-cultured with irradiation Fb as ADSC group, VEGF+ irradiation Fb was set as VEGF group, and GM-CSF+ after-radiation Fb was set as GM-CSF group. VEGF, GM-CSF and ADSCs were used to intervene in radiation injured fi broblasts, respectively, and cell apoptosis, proliferation, cell cycle, and activation of signaling pathways by WesternBlot were analyzed to verify the results. Results VEGF, GM-CSF and ADSCs intervened in radiation-injured fibroblasts, respectively, and the degrees of promoting cell proliferation, anti-apoptosis and accelerating cell cycle. Compared with the irradiation group, the diff erences were statistically signifi cant(P ＜0.05), and its degree was ADSCs, GM-CSF, and VEGF in order. The expressions of p-Akt protein and p-Erk protein in the VEGF group and GM-CSF group were up-regulated compared with the irradiation group, and the diff erence was statistically signifi cant (P ＜0.01). The expression of p-Erk protein and the degree of action were ADSCs, GM-CSF, and VEGF in order, and the diff erence was statistically signifi cant (P ＜0.01). The PI3K-Akt (phosphatidylinositol-3-kinase/protein kinase B) pathway and the MAPK (mitogen-activated protein kinase) pathway may play an important role, such as the role of VEGF and GM-CSF in the treatment of ADSCs on irradiation fi broblasts. The eff ect of VEGF and GM-CSF is much weaker than that of ADSCs, which implies that they may only be part of the cytokines during the progress of radiation-induced skin repairment. Conclusion The radiation-injured fi broblasts treated with VEGF and GM-CSF demonstrated enhanced proliferation activity, reduced apoptosis, and accelerated cell cycle progressed from G2/M to G1/S.

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更新日期/Last Update: 2023-08-28