[1]孙雯佳,吴家强,马文涓,等.SKH-1无毛小鼠光老化模型的建立及点阵CO2激光治疗效应观察[J].中国美容医学,2023,(12):56-60.[doi:10.15909/j.cnki.cn61-1347/r.006028]
　SUN Wenjia,WU Jiaqiang,Ma Wenjuan,et al.Establishment of SKH-1 Hairless Mice Photoaging Model and Its Application in Observing the Effect of Fractional CO2 Laser[J].Medical Aesthetics and Beauty,2023,(12):56-60.[doi:10.15909/j.cnki.cn61-1347/r.006028]
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SKH-1无毛小鼠光老化模型的建立及点阵CO2激光治疗效应观察()
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《中国美容医学》[ISSN:1008-6445/CN:61-1347/R]

卷:
期数:
2023年12期
页码:
56-60
栏目:
出版日期:
2023-12-10

文章信息/Info

Title:
Establishment of SKH-1 Hairless Mice Photoaging Model and Its Application in Observing the Effect of Fractional CO2 Laser
文章编号:
1008-6455（2023）12-0056-04
作者:
孙雯佳1 ; 吴家强2 ; 马文涓2 ; 任 捷2
(1.上海和睦家新城医院皮肤科 上海 201206 ;2.复旦大学附属华山医院皮肤科 上海 200040 )
Author(s):
SUN Wenjia1 ; WU Jiaqiang2 ; Ma Wenjuan2 ; Ren Jie2
(1.Department of Dermatology, Shanghai United Family Xincheng Hospital,Shanghai 201206,China; 2.Department of Dermatology,Huashan Hospital of Fudan University,Shanghai 200040,China)
关键词:
无毛小鼠; 光老化; 动物模型; 点阵激光; 组织学
Keywords:
hairless mice;  photoaging;  animal model;  fractional laser;  histology
分类号:
R758.1
DOI:
10.15909/j.cnki.cn61-1347/r.006028
文献标志码:
A
摘要:
目的：确定SKH-1无毛小鼠光老化模型的造模方法，并将该模型用于点阵CO2激光治疗效应观察。方法：将15只SKH-1 无毛小鼠分为空白组、较低剂量紫外线组和较高剂量紫外线组，紫外线照射小鼠背部皮肤，通过观察小鼠背部皮肤外观、HE 染色评估小鼠皮肤光老化程度，选定合适的紫外线照射剂量。10只光老化小鼠随机分为对照组与点阵CO2激光治疗组，治疗 前、治疗后即刻、7 d、14 d及28 d检测经皮水分丢失(Trans-epidermal water loss, TEWL)，治疗前、治疗后即刻、28 d、 56 d 行环钻活检取得皮肤组织，进行HE染色、Masson染色、阿新蓝染色及Ⅰ型、Ⅲ型胶原免疫组化染色。结果：紫外线照射 后SKH-1小鼠背部皮肤出现皮肤粗糙，横向皱纹增加，胶原纤维含量显著减少、疏松、断裂，真皮变薄、表皮增厚等光老化 表现，紫外线剂量过高可出现日光性角化甚至癌变。点阵CO2激光治疗即刻皮肤组织内可形成锥形的微小热损伤区，TEWL即 刻显著升高7倍，1～2周恢复，逐渐出现胶原新生。结论：SKH-1无毛小鼠是较理想的光老化动物实验模型，且可用于点阵激 光等美容治疗后的生理与组织学研究。
Abstract:
Objective To determine the method of establishing SKH-1 hairless mouse photoaging model, and to use this model to observe the eff ect of fractional carbon dioxide laser treatment. Methods Fifteen SKH-1 hairless mice were divided into a blank group, a low-dose-UV group and a high-dose-UV group. The dorsal skin of mice was assessed by observing the appearance and HE staining, and the appropriate UV irradiation dose was selected. Then, 10 photoaged mice were randomized into a control group and a fractional CO2 laser treatment group. Trans-epidermal water loss (TEWL) was measured before , immediately, 7 d, 14 d and, 28 d after treatment. Punch biopsy was done before and immediately, 28 days, 56 days after treatment, for HE staining, Masson staining, Alcian blue staining and immunohistochemical staining for type I and type III collagen. Results After UV irradiation, the dorsal skin of UV-irradiated SKH-1 mice showed roughness, increased transverse wrinkles, signifi cantly reduced collagen fi bers, loose and fractured collagen fi bers, thinner dermis and thickened epidermis. Excessive UV dose could cause solar keratosis or even carcinoma. Fractional CO2 laser treatment formed conical microthermal zones, with an immediate 7-fold increase in TEWL and recovery in 1-2 weeks. New collagen emerged gradually. Conclusion SKH-1 hairless mouse is an ideal experimental animal model of photoaging, and can be used for physiological and histological studies of cosmetic laser treatment.

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更新日期/Last Update: 2023-12-26