[1]秦 岭,阎海萍,尹小芳,等.脂肪干细胞与放射损伤成纤维细胞共培养后细胞因子表达的差异[J].中国美容医学,2023,(7):86-89.[doi:10.15909/j.cnki.cn61-1347/r.005762]
　QIN Ling,YAN Haiping,YIN Xiaofang,et al.Differences in Cytokines Expression after Co-Culture of Adipose Stem Cells with Irradiated Fibroblasts[J].Medical Aesthetics and Beauty,2023,(7):86-89.[doi:10.15909/j.cnki.cn61-1347/r.005762]
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脂肪干细胞与放射损伤成纤维细胞共培养后细胞因子表达的差异()
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《中国美容医学》[ISSN:1008-6445/CN:61-1347/R]

卷:
期数:
2023年7期
页码:
86-89
栏目:
出版日期:
2023-07-10

文章信息/Info

Title:
Differences in Cytokines Expression after Co-Culture of Adipose Stem Cells with Irradiated Fibroblasts
文章编号:
1008-6455（2023）07-0086-04
作者:
秦 岭1 ; 阎海萍2 ; 尹小芳1 ; 杨 超3 ; 栗颖利4 ; 李 敏5
(1.锦州医科大学解放军第960医院研究生培养基地 辽宁 锦州 121001 ;2.山东省军区济南第十一离职干部休养所 山东 济南 250013 ;3.中国人民解放军海军特色医学中心整形外科 上海 200433 ;4.解放军第960医院整形外科 山东 济南 250000 ;5.解 放军第960医院核医学科 山东 济南 250000 )
Author(s):
QIN Ling1 ; YAN Haiping2 ; YIN Xiaofang1 ; YANG Chao3 ; LI Yingli4 ; LI Min5
(1.The PLA 960th Hospital Graduate Training Base of Jinzhou Medical University,Jinzhou 121001,Liaoning,China; 2.Jinan’s Eleventh Leaving Cadre Recuperation Center of Shandong Provincial Military Region,Jinan 250013,Shandong,China; 3.Plastic Surgery of PLA Naval Medical Center,Shanghai 200433,China; 4.Department of Plastic Surgery,960th Hospital of PLA,Jinan 250000,Shandong,China; 5.Department of Nuclear Medicine, 960th Hospital of PLA,Jinan 250000,Shandong,China)
关键词:
脂肪干细胞; 成纤维细胞; 放射损伤; 细胞因子; 粒细胞-巨噬细胞集落刺激因子; 血管内皮生长因子
Keywords:
adipose derived stem cells;  fi broblast;  radiation damage;  cytokines;  granulocyte-macrophage colony stimulating factor;  vascular endothelial growth factor
分类号:
R622
DOI:
10.15909/j.cnki.cn61-1347/r.005762
文献标志码:
A
摘要:
目的：探讨脂肪干细胞（Adipose derived stem cells，ADSCs）与放射损伤成纤维细胞（Fibroblast，Fb)共培养 后细胞因子表达的差异。方法：使用剂量8 Gy的X线放射源，对大鼠的成纤维细胞进行单次照射，建立ADSCs与放射后成纤 维细胞共培养模型。分别把未干预的成纤维细胞设为空白对照Fb1组，放射后成纤维细胞设为Fb2组，ADSCs与放射后成纤 维细胞共培养组设为Fb3组，利用蛋白质芯片检测各组差异表达的细胞因子，并通过Elisa检测验证。结果：研究结果发现 Fb2 组粒细胞-巨噬细胞集落刺激因子（Granulocyte-macrophage colony stimulating factor，GM-CSF）的表达水平低于 Fb1 组（P＜0.05），Fb3组GM-CSF表达水平高于Fb2（P＜0.01）；Fb3组血管内皮生长因子（Vascular endothelial growth factor，VEGF）表达水平高于Fb2组（P＜0.05）；各组表皮细胞因子（Epidermal growth factor，EGF）和血小板衍生因子 （Platelet derived growth factor，PDGF）含量差异无统计学意义。结论：ADSCs与放射后成纤维细胞共培养后多种生长 因子、黏附因子、趋化因子上调，部分炎性因子下调；ADSCs可能主要通过VEGF和GM-CSF促进成纤维细胞放射损伤的修复。
Abstract:
Objective To investigate the diff erence in cytokines expression after co-culture of ADSCs and radiation-damaged fi broblasts. Methods An X-ray radiation source of 8 Gy was used to illuminate rat fi broblasts to establish a co-culture model of ADSCs and radiation-damaged fi broblasts. Unintervened fi broblasts were set as blank control Fb 1,radiation damaged fi broblasts as Fb2, and ADSCs with radio-injured fi broblasts as Fb3. Diff erentially expressed cytokines were detected in each group using protein microarray, which were verified by Elisa. Results The expression of granulocyte-macrophage colony stimulating factor(GM-CSF) in Fb2 group were lower than that in Fb1 group (P＜0.05),while the expression of GM-CSF in Fb3 group were higher than that in Fb2 group (P＜0.01); Moreover,the expression of vascular endothelial growth factor(VEGF) in Fb3 group were higher than that in Fb2 group (P ＜0.05). However, there was no signifi cant diff erence in epidermal growth factor(EGF) and platelet derived growth factor(PDGF) content in each group. Conclusion Some growth factors, adhesion factors, and chemokines were up-regulated while some infl ammatory factors were down-regulated after co-culture of ADSCs with radiation damaged fi broblasts. ADSCs may promote the repair of fi broblast radiation damage mainly through VEGF and GM-CSF.

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更新日期/Last Update: 2023-08-01