[1] 牛星堂,林珣珣,朱昭炜,等. 4-甲基伞形酮对瘢痕疙瘩成纤维细胞的抗炎作用及机制研究[J].中国美容医学,2020,(7):97-100.
　 NIU Xing-tang,LIN Xun-xun,ZHU Zhao-wei,et al. The Study on Anti-infl ammatory Effect and Mechanisms of 4-methylumbelliferone in Keloid Fibroblasts[J].Medical Aesthetics and Beauty,2020,(7):97-100.
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 4-甲基伞形酮对瘢痕疙瘩成纤维细胞的抗炎作用及机制研究
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《中国美容医学》[ISSN:1008-6445/CN:61-1347/R]

卷:
期数:
2020年7期
页码:
97-100
栏目:
出版日期:
2020-07-10

文章信息/Info

Title:
 The Study on Anti-infl ammatory Effect and Mechanisms of 4-methylumbelliferone in Keloid Fibroblasts

文章编号:
1008-6455（2020）07-0097-04
作者:
 牛星堂; 林珣珣; 朱昭炜; 许澍洽; 唐 庆
Author(s):
 NIU Xing-tang; LIN Xun-xun; ZHU Zhao-wei; XU Shu-qia; TANG Qing
关键词:
 [关键词]4-甲基伞形酮; 瘢痕疙瘩; 炎症因子; TLR4; NF-κB1; 作用机制
Keywords:
 Key words: 4-methylumbelliferone;  keloid;  infl ammatory factors;  TLR4;  NF-κB1;  mechanism of action
分类号:
R619+.6
文献标志码:
A
摘要:
[摘要]目的：探讨4-甲基伞形酮（4-methylumbelliferone，4MU）对瘢痕疙瘩成纤维细胞的作用及机制研究。方法：体外培
养的原代瘢痕疙瘩成纤维细胞（KFs）分别用浓度为0、0.2、0.4、0.8mM的4MU处理，采用CCK-8法和细胞划痕实验检测4MU对
KFs细胞增殖和迁移能力的影响，通过ELISA、qPCR、免疫荧光染色和Western-bloting技术检测4MU对KFs炎症相关因子表达
的影响。结果：4MU不仅抑制KFs细胞增殖迁移，而且可减少KFs对IL-6和IL-8的分泌，同时抑制HAS2和HAS3 mRNA的表达及
α-SMA、TLR4、CD44、NF-κB1、P65蛋白的合成。结论：4MU可以抑制KFs增殖和迁移，并可通过抑制NF-κB通路相关基因的
表达，减少KFs炎症因子分泌，因此，4MU有可能成为治疗瘢痕疙瘩的新药物。

Abstract:
Abstract: Objective To investigate the effect of 4-methylumbelliferone (4MU) on keloid fi broblasts (KFs). Methods Primary keloid
fi broblasts were respectively treated with 4MU at concentrations of 0, 0.2, 0.4, and 0.8 mM. The proliferation and migration ability of
KFs were detected by CCK-8 assay and cell migration assay. ELISA, qPCR, immunofl uorescence and Western-bloting techniques
were applied to study the effects of 4MU on the expression of inflammation-related genes in KFs. Results 4MU not only
inhibited the proliferation and migration of KFs, but also inhibited the secretion of IL-6 and IL-8. Additionally, 4MU inhibited
the expression of HAS2 and HAS3, and reduced the synthesis of α-SMA, TLR4, CD44, NF-κB1 and P65 proteins in KFs.
Conclusion 4MU treatment can not only inhibit the activity of KFs, but also decrease the secretion of infl ammatory by downregulating
the expression of NF-κB signal pathway. Therefore, 4MU has the potential to be a new drug for the cure of keloids.

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更新日期/Last Update: 2020-07-22