[1]钱东彬,金慧瑜.复方芪参提取物对瘢痕疙瘩成纤维细胞迁移侵袭和周期的影响[J].中国美容医学,2022,(8):108-111.
　QIAN Dongbin,JIN Huiyu.Effects of Compound Qishen Extract on Migration, Invasion and Cycle of Keloid Fibroblasts?/html>[J].Medical Aesthetics and Beauty,2022,(8):108-111.
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复方芪参提取物对瘢痕疙瘩成纤维细胞迁移侵袭和周期的影响()
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《中国美容医学》[ISSN:1008-6445/CN:61-1347/R]

卷:
期数:
2022年8期
页码:
108-111
栏目:
出版日期:
2022-08-10

文章信息/Info

Title:
Effects of Compound Qishen Extract on Migration, Invasion and Cycle of Keloid Fibroblasts?/html>
文章编号:
1008-6455（2022）08-0108-04
作者:
钱东彬1; 金慧瑜2
(1.中南财经政法大学医院皮肤科 湖北 武汉 430070 ;2.华中科技大学同济医学院附属梨园医院眼科 湖北 武汉 430077 )
Author(s):
QIAN Dongbin1 ; JIN Huiyu2
(1.Department of Dermatology,Zhongnan University of Economics and Law Hospital,Wuhan 430070,Hubei,China; 2.Department of Ophthalmology,Liyuan Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430077,Hubei,China)
关键词:
复方芪参提取物; 瘢痕疙瘩; 成纤维细胞; 增殖; 迁移; 侵袭; 细胞周期
Keywords:
compound Qishen extract;  keloid;  fi broblasts;  proliferation;  migration;  attack;  cell cycle
分类号:
R619+.6
文献标志码:
A
摘要:
目的：探讨复方芪参提取物对瘢痕疙瘩成纤维细胞迁移侵袭和周期的影响。方法：对瘢痕疙瘩成纤维细胞进行培养，使用不同浓度的复方芪参提取物（10 mg/L、20 mg/L、40 mg/L）处理瘢痕疙瘩成纤维细胞48 h，将瘢痕疙瘩成纤维细胞分为对照组与实验组，实验组分为：低浓度组，中浓度组及高浓度组，对照组不用复方芪参提取物处理。通过MTT检测复方芪参提取物对瘢痕疙瘩成纤维细胞增殖的影响；Transwell检测复方芪参提取物对瘢痕疙瘩成纤维细胞迁移、侵袭的影响；流式细胞术检测复方芪参提取物对瘢痕疙瘩成纤维细胞细胞周期的影响；Western Blot检测复方芪参提取物对瘢痕疙瘩成纤维细胞VEGF、MMP9、Cyclin D1、p53蛋白表达的影响。结果：复方芪参提取物处理瘢痕疙瘩成纤维细胞48 h后，随着复方芪参提取物浓度的增加，低、中及高浓度组细胞的增殖抑制率逐渐升高，迁移数及侵袭数逐渐降低，差异有统计学意义（P＜0.05）。与对照组相比，低、中及高浓度组在G0/G1及G2/M期的阻滞率明显上升，S期的阻滞率明显下降；且低、中及高浓度组瘢痕疙瘩成纤维细胞VEGF、MMP9、Cyclin D1、p53蛋白表达水平明显下降，差异有统计学意义（P＜0.05）。结论：复方芪参提取物能够抑制瘢痕疙瘩成纤维细胞增殖，抑制瘢痕疙瘩成纤维细胞发生侵袭与转移。
Abstract:
Objective To investigate the effects of compound Qishen extract on the migration, invasion and cycle of keloid fibroblasts. Methods Keloid fibroblasts were cultured. Keloid fibroblasts were treated with different concentrations of compound Qishen extract (10 mg/L, 20 mg/L, 40 mg/L) for 48 hours. Keloid fi broblasts were divided into control group and experimental group. The experimental group was divided into low concentration group, medium concentration group and high concentration group. The control group was not treated with compound Qishen extract. The eff ect of compound Qishen extract on the proliferation of keloid fibroblasts was detected by MTT. It was used to detect the eff ect of compound Qishen extract on the migration and invasion of keloid fi broblasts. The eff ect of compound Qishen extract on the cell cycle of keloid fi broblasts was detected by flow cytometry. The effects of compound Qishen extract on the expression of VEGF, MMP9, Cyclin D1 and p53 in keloid fi broblasts were detected by Western blot. Results It showed that 48 hours after compound Qishen extract treated keloid fi broblasts, with the increase of compound Qishen extract concentration, the proliferation inhibition rate of low concentration group, medium concentration group and high concentration group gradually increased, the differences were statistically significant (P ＜0.05). Compared with the control group, with the increase of the concentration of compound Qishen extract, the number of cell migration and cell invasion decreased gradually in low concentration group, medium concentration group and high concentration group (P＜0.05). Compared with the control group, the block rate of low concentration group, medium concentration group and high concentration group increased significantly in G0/G1 and G2/M phase, decreased significantly in S phase (P ＜0.05). Compared with the control group, the expression levels of VEGF, MMP9, Cyclin D1 and p53 in keloid fibroblasts in low concentration group, medium concentration group and high concentration group decreased significantly (P ＜0.05). Conclusion Compound Qishen extract can inhibit the proliferation of keloid fi broblasts, inhibit the invasion and metastasis of keloid fi broblasts

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更新日期/Last Update: 2022-08-25