[1]邓雨萌,岑蕊言,王利群,等.FOXP3减少瘢痕疙瘩成纤维细胞的胶原分泌及相关机制初探[J].中国美容医学,2023,(5):90-95.
　DENG Yumeng,CEN Ruiyan,WANG Liqun,et al.A Preliminary Investigation on the Reduction of Collagen Secretion by FOXP3 in Keloid Fibroblasts and Related Mechanisms[J].Medical Aesthetics and Beauty,2023,(5):90-95.
点击复制

FOXP3减少瘢痕疙瘩成纤维细胞的胶原分泌及相关机制初探()
分享到：

《中国美容医学》[ISSN:1008-6445/CN:61-1347/R]

卷:
期数:
2023年5期
页码:
90-95
栏目:
出版日期:
2023-05-10

文章信息/Info

Title:
A Preliminary Investigation on the Reduction of Collagen Secretion by FOXP3 in Keloid Fibroblasts and Related Mechanisms
文章编号:
1008-6455（2023）05-0090-05
作者:
邓雨萌1 ; 岑蕊言1 ; 王利群1 ; 何咏磬1 ; 杨桂红1 ; 邓 军2 ; 赵 艳3 ; 唐 辉1 ; 雷 霞1
(1.陆军军医大学大坪医院皮肤科 重庆 400042 ;2.重庆市玛恩医疗美容医院 重庆 401121 ;3.陆军医科大学大坪医院陆军 特殊职业疾病防控研究室 重庆 400042 )
Author(s):
DENG Yumeng' target="_blank" rel="external"> sans-serif; font-size: 10.5pt;">DENG Yumeng1 ; CEN Ruiyan1 ; WANG Liqun1 ; HE Yongqing1 ; YANG Guihong1 ; DENG Jun2 ;  ZHAO Yan3 ; TANG Hui1 ; LEI Xia1
关键词:
瘢痕疙瘩; 成纤维细胞; 叉头样转录因子3; 转化生长因子-β1; 胶原沉积
Keywords:
keloid;  fi broblast;  forkhead-like transcription factor 3;  transforming growth factor 1;  collagen deposition
分类号:
R751.05
文献标志码:
A
摘要:
目的：探讨在瘢痕疙瘩成纤维细胞（Keloid fibroblasts,KFB）中叉头样转录因子3（Forkhead-like transcription factor 3,FOXP3）对真皮胶原蛋白沉积及纤维化的影响及相关机制。方法：免疫组化检测瘢痕疙瘩组织中I型胶原蛋白 (Collagen type I,COL1) 、Ⅲ型胶原蛋白(Collagen type Ⅲ,COL3)、IL-6、TGF-β1、FOXP3的表达。体外分离培养KFB， 并采用腺病毒过表达FOXP3或给予TGF-β1抑制剂处理。通过Western blot检测对照组、过表达组以及对照组和抑制剂组的 COL1 、COL3的表达，ELISA检测TGF-β1、IL-6的表达，Masson染色观察胶原沉积情况。结果：与对照组相比，实验组中的 COL1 、COL3、IL-6、TGF-β1蛋白水平升高，FOXP3蛋白水平降低。经过表达FOXP3或TGF-β1抑制剂处理后，KFB的TGF-β1和 IL-6 表达降低，胶原蛋白沉积减少。结论：在KFB中FOXP3可能通过抑制TGF-β1的表达，减少组织纤维化和胶原蛋白沉积。
Abstract:
Objective To investigate the eff ect of Forkhead-like transcription factor 3 (FOXP3) on dermal collagen deposition and fi brosis in keloid fi broblasts（KFB）and related mechanisms. Methods Immunohistochemistry was performed to detect the expression of Collagen type I (COL1), Collagen type III (COL3), IL-6, TGF-β1, and FOXP3 in keloid tissues. KFB were isolated and cultured in vitro and treated with adenoviral overexpression of FOXP3 or given TGF-β1 inhibitors. The expression of COL1 and COL3 in control and overexpression groups as well as control and inhibitor groups were detected by Western blot, TGF-β1 and IL-6 by ELISA, and collagen deposition was observed by Masson staining. Results Compared with the control group, the levels of COL1, COL3, IL-6, and TGF-β1 proteins were increased and FOXP3 protein levels were decreased in the experimental group. After treatment with expression of FOXP3 or TGF-β1 inhibitor, TGF-β1 and IL-6 expression in KFB was decreased and collagen deposition was reduced. Conclusion In KFB FOXP3 may reduce tissue fi brosis and collagen deposition by inhibiting the expression of TGF-β1.

相似文献/References:

[1]倪 娜,周炳荣,马委委,等. miRNA-769-5p上调对正常人成纤维细胞生物活性的影响[J].中国美容医学,2018,(04):109.
　NI Na,ZHOU Bing-rong,MA Wei-wei,et al. Effect of miRNA-769-5p on Biological Activity of Normal Human Fibroblasts[J].Medical Aesthetics and Beauty,2018,(5):109.
[2]任润健,方 磊,贾传龙,等. PDGF-BB对UVB诱导的光老化成纤维细胞中ROS的影响[J].中国美容医学,2018,(05):109.
　REN Run-jian,FANG Lei,JIA Chuan-long,et al. Effect of PDGF-BB on Reactive Oxygen Species in UVB-induced Skin Fibroblasts[J].Medical Aesthetics and Beauty,2018,(5):109.
[3]朱明宇,李雨珊,王志国,等. 机械牵张力加载时间对诱导正常皮肤成纤维细胞向增生性瘢痕成纤维细胞转化过程的影响研究[J].中国美容医学,2018,(06):55.
　ZHU Ming-yu,LI Yu-shan,WANG Zhi-guo,et al. The Study of the Effect of Mechanical Stretch Loading Time on the Transformation of Normal Skin Fibroblasts to Hypertrophic Scar Fibroblasts[J].Medical Aesthetics and Beauty,2018,(5):55.
[4]王春贵,张宝林.手术切除联合放疗与博来霉素注射治疗耳廓瘢痕疙瘩疗效对比[J].中国美容医学,2018,(06):71.
　WANG Chun-gui,ZHANG Bao-lin. Comparison of Curative Effect between Combined Radiotherapy and Bolamycin Injection after Auricle Keloid Resection[J].Medical Aesthetics and Beauty,2018,(5):71.
[5]王文婷,刘 涛,宋海峰,等. 浅层电子线放射治疗背部瘢痕疙瘩的体外实验与临床疗效观察[J].中国美容医学,2018,(07):87.
　WANG Wen-ting,LIU Tao,SONG Hai-feng,et al. Observation the In Vitro Experiment and Clinical Effectof Postoperative Shallow Electron Beam Radiotherapy in the Treatment of Prothorax Keloid[J].Medical Aesthetics and Beauty,2018,(5):87.
[6]楚菲菲,王大雷,闫 伦,等.阻断Notch信号的角质形成细胞对成纤维细胞增殖及分泌功能的影响研究[J].中国美容医学,2018,(08):73.
　CHU Fei-fei,WANG Da-lei,YAN Lun,et al.Differences of Cell Proliferation and Secretion in Fibroblasts Co-culture with Notch Signaling Occured Keratinocytes[J].Medical Aesthetics and Beauty,2018,(5):73.
[7]吕春风,刘胜达,种 红,等. 皮瓣移植术后应用高压氧治疗对皮瓣存活的影响研究[J].中国美容医学,2019,(01):31.
　LYU Chun-feng,LIU Sheng-da,CHONG Hong,et al. Effect of Hyperbaric Oxygen Therapy on the Survival of Skin Flap after Flap Transplantation[J].Medical Aesthetics and Beauty,2019,(5):31.
[8]王 琳,唐海玲,胡志坤. 硬皮病及瘢痕疙瘩与硬化萎缩性苔藓患者LIGHT及其受体LTβR和HVEM病理生理学差异分析[J].中国美容医学,2019,(02):80.
　WANG Lin,TANG Hai-ling,HU Zhi-kun. Difference Analysis of Pathophysiological Significance between Light and ItsReceptor LTβR and Hvem in Patients with Scleroderma,Keloid and SclerosingAtrophic Moss[J].Medical Aesthetics and Beauty,2019,(5):80.
[9]孙 楠,吴 艳,姜 莉. 水母雪莲多糖对中波紫外线诱导的皮肤成纤维细胞凋亡的研究[J].中国美容医学,2019,(03):100.
　SUN Nan,WU Yan,JIANG Li. Study on the Apoptosis of Skin Fibroblasts Induced by UVB in SaussureaMedusa Maxim Polysaccharides[J].Medical Aesthetics and Beauty,2019,(5):100.
[10]孙 楠,吴 艳,姜 莉. 水母雪莲多糖对中波紫外线诱导的皮肤成纤维细胞凋亡的研究[J].中国美容医学,2019,(03):100.
　SUN Nan,WU Yan,JIANG Li. Study on the Apoptosis of Skin Fibroblasts Induced by UVB in SaussureaMedusa Maxim Polysaccharides[J].Medical Aesthetics and Beauty,2019,(5):100.
[11]徐志山,王洪一,钟黎明,等. 苦参碱对瘢痕疙瘩成纤维细胞生长的抑制作用实验研究[J].中国美容医学,2018,(10):137.
　XU Zhi-shan,WANG Hong-yi,ZHONG Li-ming,et al. Inhibitory Effect of Matrine on the Growth of Keloid Fibroblasts[J].Medical Aesthetics and Beauty,2018,(5):137.
[12]梁仲尧,岑 瑛.二肽基肽酶-4在瘢痕疙瘩中的表达情况研究[J].中国美容医学,2022,(1):87.
　LIANG Zhongyao,CEN Ying.Study on the Expression of Dipeptidyl Peptidase-4 in Keloids[J].Medical Aesthetics and Beauty,2022,(5):87.
[13]钱东彬,金慧瑜.复方芪参提取物对瘢痕疙瘩成纤维细胞迁移侵袭和周期的影响[J].中国美容医学,2022,(8):108.
　QIAN Dongbin,JIN Huiyu.Effects of Compound Qishen Extract on Migration, Invasion and Cycle of Keloid Fibroblasts?/html>[J].Medical Aesthetics and Beauty,2022,(5):108.
[14]王 猛,王 品,黄 谦,等.siRNA干扰LOXL2基因在瘢痕疙瘩成纤维细胞中的相关性研究[J].中国美容医学,2022,(10):112.
　WANG Meng,WANG Pin,HUANG Qian,et al.Study on the Correlation of siRNA Interference with LOXL2 Gene in Keloid Fibroblasts[J].Medical Aesthetics and Beauty,2022,(5):112.
[15]贾文斌 综述,谭 谦 审校.病理性瘢痕的非手术治疗新进展[J].中国美容医学,2023,(1):183.
　JIA Wenbin,TAN Qian.New Progress in Non-surgical Treatment of Pathological Scar[J].Medical Aesthetics and Beauty,2023,(5):183.
[16]潘 铃 综述,李冬青 吴信峰 审校.长链非编码RNA在皮肤纤维化疾病中的研究进展[J].中国美容医学,2023,(2):186.
　PAN Ling,LI Dongqing,WU Xinfeng.The Research Progress of Long non-coding RNA in Skin Fibrotic Diseases[J].Medical Aesthetics and Beauty,2023,(5):186.
[17]詹明峰,张文娟,孙士芳,等.LNCRNA8975-1对瘢痕疙瘩成纤维细胞增殖周期和迁移的影响及其机制[J].中国美容医学,2023,(8):35.[doi:10.15909/j.cnki.cn61-1347/r.005829]
　ZHAN Mingfeng,ZHANG Wenjuan,SUN Shifang,et al.Effect of LNCRNA8975-1 on Proliferation Cycle and Migration of Keloid Fibroblasts and Its Mechanism[J].Medical Aesthetics and Beauty,2023,(5):35.[doi:10.15909/j.cnki.cn61-1347/r.005829]
[18]贾尚洁 综述,陈 涛 审校.miRNA在瘢痕疙瘩中的研究现状[J].中国美容医学,2024,(2):195.[doi:10.15909/j.cnki.cn61-1347/r.006163]
　IA Shangjie,CHEN Tao.Research Status of miRNA in Keloids[J].Medical Aesthetics and Beauty,2024,(5):195.[doi:10.15909/j.cnki.cn61-1347/r.006163]
[19]林 颜,阮树斌,陈晓东,等.干扰hsa_circ_0013958/miR-637对人瘢痕疙瘩成纤维细胞增殖、迁移和侵袭的影响[J].中国美容医学,2024,(4):54.
　LIN Yan,RUAN Shubin,CHEN Xiaodong,et al.Effect of Interference hsa_circ_0013958/miR-637 on the Proliferation, Migration and Invasion of Human Keloid Fibroblasts[J].Medical Aesthetics and Beauty,2024,(5):54.

更新日期/Last Update: 2023-06-16